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Whole-cell binding assay For GIPR, CHO-K1 cells were cultured in F-12 medium with 10% FBS and seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer)

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These observations suggest that in terms of stress tolerance, the production of exopolysaccharides may serve an on/off function rather than a gradient of tolerance, and that the increased production of exopolysaccharides due to pH stress may have another role

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The above results would collectively suggest that BoCA, in part, leads to gut microbiome reorganization, which increased gut-host production of PAGln