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10m-sections were cut on a cryostat (Leica Biosystems) and collected on SuperFrost Plus slides, then air-dried at room temperature for 1 h followed by incubation at 40 C for 5 min

As a triple receptor agonist targeting GLP-1, GIP, and glucagon pathways simultaneously, retatrutide represents one of the most advanced incretinomimetic compounds under investigation for metabolic and obesity research

Semaglutide is a GLP-1 agonist, a hormone that helps regulate hunger, control insulin release, and slow digestion

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