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GST-xCTD repeats GST-2xCTD repeats, GST-4xCTD repeats, and GST-10xCTD repeats were expressed in BL21 Star (DE3) cells grown in Overnight Express Instant TB Medium (EMD Millipore) at 37C and induced at an OD 600 of 1.0 by decreasing the temperature to 18C and growing for an additional 16 hours

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