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The system must be complied with regulatory standards

For each sample, mRNA was purified from 1 g of total RNA using oligo(dT)-based magnetic bead isolation (Poly(A) mRNA Magnetic Isolation Module, New England Biolabs), following the manufacturers protocol

The investigators compared people who had used these drugs with clinically similar patients who had not received them, then assessed whether either group was more or less likely to develop substance use disorders