Reported and observed concerns include: Gastrointestinal distress (nausea, vomiting, diarrhea, constipation) Reduced nutrient intake and absorption, increasing micronutrient deficiencies Lean muscle loss, especially when weight loss is rapid Fatigue and low energy, often overlooked but frequently reported Pancreatic and gallbladder stress in susceptible individuals These side effects are not simply inconveniences they reflect underlying cellular stress during rapid metabolic change
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It has no preservative agent
Briefly, to separate epidermis from dermis, whole skin from mice at postnatal day (P)0-P3 were incubated in a solution of 5 mg/ml Dispase II (#D4693, Sigma-Aldrich, USA) diluted in RPMI medium (Gibco, USA) supplemented with 10% fetal calf serum (FCS, #S0615, Sigma-Aldrich, USA), penicillin (100 U/ml), streptomycin (100 g/ml), 100 nM sodium pyruvate, and 10 mM non-essential amino acids (all Gibco, USA) at 4 C overnight