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Material and methods Cell culture Human epithelial cancer cell lines from different head and neck regions UM-SCC-1 (floor of mouth), WSU-HN6 (tongue), and WSU-HN30 (pharyngeal) were cultured in Dulbeccos modified Eagles Medium (DMEM) supplemented with 10% fetal bovine serum, 1% L-glutamine, and 1% antibioticantimycotic (Gibco)

The problem with smokeless tobacco products The main problem with smokeless tobacco products is that they can irritate and damage the oral mucosa, or lining of the oral cavity

Vaping is now being advertised as healthier and a better alternative, similar to how smoking once was advertised

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