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The CBS OPT and L2HGDH OPT were then constructed in pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro (#73037, Addgene), with the Nluc-P2A-coGFP genes removed

found that dietary Gln supplementation can elevate Gln levels in the TME, decrease the expression of immunosuppressive genes such as Ctla4 and Nrp1 in Tregs, inhibit Treg differentiation, and consequently enhance the tumor immune response (52)

10.1111/j.1471-4159.2005.02999.x 121 NgoS

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Therefore, many research teams prioritize organization, procedural consistency, and standardized evaluation methods when conducting ongoing laboratory investigations