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Total RNA was extracted using TRIzol (Invitrogen, California, USA) and isolated using Oligotex mRNA Mini kit (Qiagen, Duesseldorf, Germany) according to the manufacturers instructions

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Yes, variants in the GLP1R gene (e.g., rs6923761) have been associated with differences in weight loss response to GLP-1 medications in clinical studies

HIF-1 and HIF-2 have been identified as regulators of ferroptosis in renal clear cell carcinoma cells [107]

There are also uncommon variations of male pattern baldness, such as overall thinning of the scalp without a recession of the hairline or hair loss in the crown area without a hairline recession