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A pilot clinical trial on l-carnitine supplementation in combination with motivation training: effects on weight management in healthy volunteers
The blots were incubated with primary antibodies against PGC-1 (Chemicon International, Temecula, CA) and AMP-activated protein kinase (AMPK) (Cell Signaling Technology, Beverly, MA), and the reaction products were visualized using a horseradish peroxidase-conjugated secondary antibody (Invitrogen, Carlsbad, CA) and enhanced chemiluminescence (Chemi-Lumi One Super, Nakarai Tesque, Kyoto, Japan) (Additional file 1: Figure S1)
The treatment was 1,000 g/day of cyanocobalamin
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