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It is to be noted that the BCKDH reaction is essentially irreversible in mammalian cells such that BCKA synthesis depends on essential amino acids, BCAAs, due to the lack of BCKAs de novo synthetases

In summary the GS-NEM adduct was found to be stable under the conditions of the assay (temperature, time and concentration)

Xu T, Yang Y, Chen Z, Wang J, Wang X, Zheng Y, et al

In the present study, PHH not only indicated an upregulation of glutathione upon NFT expose but also confirmed an elevated resilience of NFT pre-treated cells toward other stressors