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Each hGM group was established by gavaging germ-free mice with 200 L of human fecal suspension, which was prepared by using a 1 g aliquot of feces diluted in 10 mL of reduced phosphate-buffered saline (supplemented with 0.05% l-cysteine) in an anaerobic chamber

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Real TimePCR and melting curve analysis were done as described before 58