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Briefly, flash-frozen tissue samples were homogenized in ice-cold PBS containing 2 mM EDTA (1 ml PBS/EDTA per 10 mg tissue) using a Dounce homogenizer

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From Pubchem Synonyms: Insulin-like growth factor long chain R3, Long-(arg3)insulin-like growth factor-i Molecular Formula: C400H625N111O115S9 Molecular Weight: 9117.60 g/mol CAS number: 143045-27-6 PubChem CID: 381123731 IGF-1 LR3 retains the pharmacological properties of IGF-1 and binds to insulin-like growth factor-1 receptor