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While freezing doesnt chemically destroy benzyl alcohol, the freeze-thaw cycle can cause the preservative to separate from the water

To measure the level of total carnitine, the obtained supernatant was mixed with 1 M KOH in an Eppendorf tube and kept at 37 C for 20 min for the degradation of acylcarnitines

Oxidized proteins and a reduction in antioxidant levels contribute to the development of liver diseases

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The pattern is consistent