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We also sought comment on our intent to release the technical specifications of non-MIPS measures via notice-and-comment rulemaking, and if it allows adequate time for ASM participants to make any needed adjustments to data collections systems or practice workflows

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Finally, 100 L OPD chromogen substrate containing H 2 O 2 were added to each well and the plates incubated, protected from the light, for 15 min and the reaction was stopped by the addition of 50 L 2 M H 2 SO 4