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the original rodent study used 5 mg/kg, which when scaled allometrically to human equivalents suggests doses in the 5-10 mg range for a 70 kg adult, though allometric scaling from mice to humans is an imprecise science

If your 5mg vial is 99.95% pure but only contains 3.89mg of product, you have a very pure but significantly undersized sample

The 1-methylnicotinamide product of NNMT may itself have biological activities (including potential insulin resistance-promoting effects), and reducing its production through NNMT inhibition may contribute to metabolic improvements

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Dose and timing should be set against a confirmed deficiency, not by how you feel on any given week