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For instance, we introduced the Gly-to-Leu mutation at position 2 of Go-Nt 7 -GFP as GPS-Lipid predicted very high likelihood for S-palmitoylation at Cys3 in the mutant

These animals were weaned as per normal at 3 weeks of age onto normal drinking water or water containing NMN (2 g/L)

A mouse model of EMs was then successfully established, and animals were divided into seven groups: erastin, Ferrostatin-1, DMSO, corn oil, low-dose GSTO-IN-2, medium-dose GSTO-IN-2, and high-dose GSTO-IN-2 (Fig