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Nevertheless, current nucleic acid methods exhibit representatively the restricted sensitivity for living cell imaging and intracellular analysis 29,30,31

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Sample collection Blood samples were collected via jugular venipuncture at day 0 (mean environmental temperature 35.3 0.7C), 28 (25 4.1C), and 56 (12.6 3.1C) prior to the morning feeding into tubes with 1) clot activator for collection of serum (BD Vacutainer , Franklin Lakes, NJ)