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doi: 10.3389/fnut.2022.853846 Received 13 January 2022 Accepted 14 February 2022 Published 04 April 2022 Volume 9 - 2022 Edited by Minhao Xie, Nanjing University of Finance and Economics, China Reviewed by Lanting Zeng, South China Botanical Garden (CAS), China

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After 10-15 days of culture, the cells were fixed with 4% paraformaldehyde for 30 min after washing the medium with phosphate buffered saline (PBS), then stained with 0.1% crystal violet for 30 min, and the dye was washed and dried with PBS buffer, and the images were acquired after drying