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The second step involves N-acetyl serotonin becoming methylated by acetyl serotonin o-methyltransferase (ASMT) to become melatonin ( in utero and in adult life ( Figure 2 4.2 Melatonin receptors in the placenta Melatonins effects are mediated by two receptors, MT1 (Mel1a) and MT2 (Mel1b) ( Figure 3 ) ( i ), stimulatory G-protein (G s ), and guanine-nucleotide binding protein (G q ) ( s protein stimulates the increase of cyclic AMP (cAMP) levels by activating adenylyl cyclase, while G i inhibits cAMP levels ( q induces the expression of phospholipase C, which stimulates protein kinase C (PKC) and calcium ( q , which enables the production of protein kinase C (PKC) and the increase of calcium associated with IP 3 ( Figure 3 4.3 Melatonin in the immune microenvironment of the placenta Pregnancy is associated with immunological changes that result in the fetus avoiding immune rejection by the mother

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