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Western blots Adult retinal tissue pooled from 1-3 fish (tissue from 2-6 eyes) were homogenized in Pierce RIPA Buffer (ThermoFisher) lysis reagent (1 L/mg tissue) supplemented with 1X Halt Protease Inhibitor Cocktail (ThermoFisher) using a sterile microcentrifuge pestle and cordless pestle motor (VWR)

BPC-157 possibly resulted in an upregulation of VEGF-a expression and acceleration of vascular tube formation

1 Important Note on the Evidence Base Documented Research Integrity Issues This compound carries the heaviest evidence-base disclosure in this catalog

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The optimal dosing, administration route, and cycling protocol for humans remain uncertain