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This notion was confirmed using metabolic cages, revealing that GLP-1MK-801 counteracted the decrease in energy expenditure that is engaged by calorie-restricted mammals as an adaptive starvation response to protect the organism from an excessive loss of body weight and fat mass (Fig

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Filtered viral supernatant was then concentrated by Lenti-X Concentrator (#631232, Takara) following the manufacturers instruction and the virus pellet was resuspended in 1/100th of the original volume using cold DMEM

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