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Numerous in vitro , in vivo , and clinical studies have been conducted to determine the immunomodulatory effects of Z

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According to the latest guidelines from the American Veterinary Medical Association (50) and guidelines adopted by institutions such as Boston University (51) and the University of Maryland (52), the rats were anesthetized by intraperitoneal injection of ketamine (80 mg/kg) and xylazine (10 mg/kg), in compliance with approved ethical standards (53,54)