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Next, the apo-enzyme was eluted using GST elution buffer (50 mM Tris, 10 mM methylglutathione, pH 8) before relevant fractions were combined according to their A 280nm

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Because GHK is naturally present in the body, declines with age, has an extensive safety record, and shows consistent regenerative and protective effects across many tissues (skin, lung, nerve, connective tissue, and more), the study proposes that GHK represents a promising therapeutic and preventive compound