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Ca 2+ release Cells were plated in Matrigel-coated dishes (MatTek, Ashland, MA) 13 days prior to use and loaded with fura-2 AM by incubation for 2 h in a medium containing 25 mM Hepes (pH 7.4), 120 mM NaCl, 4.0 mM KCl, 1 mM CaCl 2 , and 1 mM MgCl 2 , and 14 mM glucose at room temperature
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