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After incubation of Glu or erastin for 8 h, PI and Hoechst 33342 at the concentration of 5 g/ml were treated for 10 min

Thats a genuinely interesting mechanistic story

The risk of liver damage necessitates careful monitoring and management strategies to balance efficacy with safety

However, these in vitro studies often use relatively high Mn concentrations (up to 1 mM), exceeding physiological brain Mn levels, but acute culture systems generally require higher doses to reveal mechanistic effects in the absence of the physiological environment

The issue to consider is that athlete in many sports have to ensure they abide by NGB rules ( eg