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Ishikawa, HEC-1A, HEC-1B, AN3CA, and SPEC2 cells were cultured in DMEM/F12 medium supplemented with 10% (v/v) FBS

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Freshly made solutions of equal volume 5,5-dithio-bis(2-nitrobenzoic acid) and glutathione reductase with concentrations of 0.33 g l 1 and 1.67 units per ml, respectively, were mixed with lysate to a final volume of 140 l