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Samples were incubated at room temperature for 1 h, vortexing intermittently, and then quenched by the addition of hydroxylamine (5 L of 5% w/v in HPLC water per sample) and incubated for 15 min at room temperature

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The interaction between Tyr 50 of wild GSTP1 and JNK has two pi cation interactions whereas only one pi cation interaction showed between mutant GSTP1 and JNK