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Chronic hyperglycemia is responsible for many complications, including cardiovascular disease, nerve damage (neuropathy), kidney damage (nephropathy), eye disease, unintentional weight loss (due to metabolism of fat and muscle, as cells do not have access to glucose to metabolize), and diabetic ketoacidosis

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After 24 h, the HFF-1 cells in the well plates were imaged using phase contrast microscopy (Zeiss, Axio, Germany) for growth appearance, adherence and confluence

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