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CJC-1295 and Ipamorelin work through different but complementary pathways

For GSK3 overexpression, cardiomyocytes were transfected with a plasmid encoding constitutively active GSK3 using Lipofectamine 3000 reagent (Invitrogen), following the manufacturers instructions

Its mechanism NNMT inhibition leading to AMPK activation directly improves insulin sensitivity, as demonstrated in the 2021 Nature Metabolism study where treated mice showed reduced hepatic steatosis and improved glucose tolerance