In agreement with in vitro findings, DMF treatment induced the expression of Cbs, Ahcy, and NRF2 targets such as Gclc, Gclm, and Slc7a11, but not that of Cse (Fig
Increased vascularity
This step involves the demultiplexing of cells using cell barcodes, the alignment of reads to GRCm39 reference genome, removal of empty droplets, cell debris and low-quality cells
Sample buffer containing SDS, glycerol, Tris-HCl pH 6.8, and bromophenol blue was added to each sample to the following final concentrations: 2% SDS, 10% glycerol, 50 mM Tris-HCl, and 0.02% bromophenol blue
( E H ) MDA-MB-231 cells transfected with siCTR or 2 different combination pools of 4 individual siRNA for FADS1 (siFADS1_pool1 and pool2) and FADS2 (siFADS2_pool1 and pool2) and assessed by qRT-PCR ( E ) were treated ON with 0.25 M RSL3 ( F ) or 2 h with 1 M RSL3 ( G , H ) and assayed for cell survival ( F ), confocal analysis ( G ), and cytofluorimetric analysis ( H ) to measure lipid peroxidation