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a) dissolving Semaglutide in a buffer solution, b) subjecting the solution to RP-HPLC purification by using buffer solution as mobile phase A and acetonitrile optionally in mixture with alcohol solvent as mobile phase B, c) subjecting the solution to RP-HPLC by using buffer solution as mobile phase A and acetonitrile as mobile phase B

The 1:10 dilution of obtained cecal conditional media (CCM) was used to treat cells

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