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Ubezio, F
Following the incubation period, cochlear samples are fixed in 4% paraformaldehyde for a minimum of 30 minutes after treatment then permeabilized in 0.5% Triton in PBS (PBST) for 1 hour, followed by overnight incubation at 4 C
these results suggested the impairment of autophagic flux in RTCs
Garcia-Prat, L., Munoz-Canoves, P
There has been a concern that high doses can raise the level of a substance called TMAO (linked in some studies to a greater risk of heart disease), but the evidence is not clear-cut, and the doses used in most studies (about 2,000 mg a day) are considered safe
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