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In brief, after 8 h of preoperative water and food fasting, adult male SpragueDawley (SD) rats weighing 275 25 g (purchased from Spearfish, China) were sedated in an induction chamber with isoflurane (oxygen flow rate of 3 L/min, 5 mL/kg) and then injected with an intramuscular injection of 1% pentobarbital (Wampanoag, P11011, Germany) at a concentration of 0.5 mL/100 g, and then observed for 35 min until the rats entered deep anesthesia

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To effectively defend against these charges, you need to understand blood alcohol content, and how it relates to your level at the time of driving, not just at the time of the test

Primer sequences are provided in Data sheet 1 : Supplementary Table S1 (synthesized by Sangon Biotech Co., Ltd., Shanghai)