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In brief, hPSCs were plated at a density of 5000 cells/well in a 24-well plate in mTeSR1 medium (Stem Cell Technologies, Vancouver, BC, Canada) + 10 M Y27632 (LC Laboratories, Woodburn, MA) on glass plates (LabTek) coated with 3% GelTrex (Thermo Fisher Scientific, USA) (Day-3)

doi: 10.1161/01.CIR.0000112605.43318.CA