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Once concentrated, the folded proteins cK1-1f and cK1-2f were dialyzed twice (respectively 1 and 18 h) at 4 C against 10 mM PBS (pH 7.4) containing 10% in vol of glycerol (2 0.3 L)

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Eluates were dried and then resolubilized in 100 L of 10% ACN prior to LC-MS/MS analysis