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Serum cytokine assays The serum IL-1, IL-6 and TNF- levels were detected by ELISA (Abebio, Wuhan, China) following the manufacturers instructions

After 4 sessions: visible reduction in dark spots, better balance

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Some studies have proposed that inhibition of autophagy can affect the progression of OA by causing chondrocyte apoptosis through mitochondrial dysfunction, so the knockdown of the mTOR signaling pathway can improve the balance between synthesis and degradation of chondrocyte extracellular matrix in osseous joints (128)

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