Collected drops were subjected to an initial denaturation at 98 C for 1 min and then cycled for two sets of 25 PCR cycles ( first cycle: 98 C for 15 s, 50 C for 20 s, 72 C for 1 min
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However, this leads to a different conformational change as compared to 17-estradiol and the hydrophobic pocket is not sealed by helix-12 8