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A hundred l PreScission protease (GE Healthcare Life Sciences) solution containing 50 mM Tris (pH 8.0), 100 mM NaCl, 1 mM EDTA, 1 mM DTT was added to the column and incubated for 12~16 hr at 4 C, and then 3MST, which had been excised from GST-fusion, was recovered by centrifugation at 735 g for 1 min at room temperature

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