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5-Amino-1MQ was identified as a potent NNMT inhibitor in work published by the Bhatt Lab and collaborators, with subsequent preclinical studies demonstrating that NNMT inhibition in diet-induced obese mice reduced body weight, decreased adipose tissue mass, and improved metabolic parameters including insulin sensitivity and plasma lipids

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The compound's selectivity profile minimizes concerns about off-target effects, but investigators should consider potential interactions with other NAD+-modulating interventions or compounds that affect methylation metabolism