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The next day cells were incubated with SNAP-Surface549 (New England Biolabs) in complete culture medium for 20 min at 37 C and washed 3 5 min using HEPES-bicarbonate buffer, leading to labeling of ~80% of surface GLP1R required for single-molecule analysis

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Tirzepatida , ao combinar GIP com GLP1, elevou a perda mdia para cerca de 26%, aproximando-se dos efeitos observados na cirurgia baritrica

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