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doi:10.3390/ijms22158199

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While the exact timeline varies for everyone, many individuals with a genuine B12 deficiency begin to experience noticeable improvements in their energy levels within a few days to a week after their first injection

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If you are taking metformin, your healthcare provider may recommend additional vitamin B-12 supplementation, especially if you are on a high dose of metformin or if you have other risk factors for vitamin B-12 deficiency