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They sprayed the ISG and drug solution (control) into the nostril using a VP3 spray device (Aptar, Crystal Lake, IL, USA) and visualized drug deposition for 20 min

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Accompanying with that, both ketamine and sevoflurane induced upregulation of RASD1, which could form a ternary complex with PAP7 and DMT1 and thus enhance the ability of NMDAR to activate DMT1 for iron uptake [8, 37]