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As mentioned, freezing a liquid peptide solution is a death sentence

RINm5F cells were seeded in XF 24-well cell culture microplates (Seahorse Bioscience, Billerica, MA) at 1.0 10 5 cells/well in 100 l complete RPMI-1640 growth medium, and then incubated at 37 C/5% CO 2 for 18-20 h