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DCFH-DA fluorescence staining demonstrated significantly elevated ROS levels in tumor cells treated with NCs, inducing mitochondrial membrane potential depolarization (JC-1), GSH depletion (ThiolTracker TM Violet), and extensive apoptosis, as observed by a cell apoptosis staining kit (Annexin V-FITC/PI) and flow cytometry

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Moderate symptoms might include persistent nausea that affects appetite or recurring diarrhea that requires schedule adjustments

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