At 8 months, tGSH and GSH from the whole homogenate were significantly decreased in mutants compared with controls (Extended Data Fig
BxPC-3 and HPDE6-C7 cells in the logarithmic growth phase were detached with trypsin and resuspended in RPMI-1640 medium containing 10% fetal bovine serum to form a single-cell suspension at a density of 5 10 4 cells/mL
In the early stage of tumor colonization or growth, activated immune cells contribute to a tumor-suppressive inflammatory microenvironment that hinders tumor development, whereas long-term persistent antigenic stimulation, the activation of immunosuppressive cells and metabolic stress prompt the tumor to escape from the surveillance of the immune system and continue to grow, which is known as immune escape of the tumor (70,71)
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