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For a low-budget talkfest, you'd expect a center-front emphasis and in fact, after the UA logo at the beginning, you can give your surround speakers the night off, as only the barest bits of ambiance sporadically show up in back and only audible with your ear to the speaker

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Absorbance (optical density, OD) was subsequently measured at 550 nm by means of an xMark microplate spectrophotometer (Bio-Rad, Mississauga, ON, Canada)